目的 摸清柳蒿芽脂溶性部位中有效成分相对含量。方法 采用气相色谱法同时测定柳蒿芽脂溶性部位中(+)-(S)-dihydro-ar-curcumene(1)、(+)-(S)-ar-curcumene (2)、(+)-(S)-dihydro-ar-turmerone(3)、(+)-(S)-ar-turmerone(4)、丁香酚(5)、棕榈酸(6),亚油酸(7)、亚麻酸(8)、12,13-环氧亚麻酸(9)、油酸(10)和chamazulene(11)的含量。结果 在选择的气相色谱条件下,被检测化合物1、2、4、5、8的线性范围均为5.0~100.0 μg·mL-1(r＞0.999 3),而3、6、7、9、10、11的线性范围均为1.0~50.0 μg·mL-1(r＞0.999 4)。化合物1~11平均回收率分别为97.8%(RSD=1.7%)、96.3%(RSD=1.8%)、97.0%(RSD=1.8%)、96.7%(RSD=1.7%)、98.2%(RSD=1.8%)、96.8%(RSD=1.9%)、96.3%(RSD=1.8%)、97.3%(RSD=1.9%)、97.1%(RSD=2.0%)、97.2%(RSD=1.7%)和97.7%(RSD=1.7%)。结论 建立了柳蒿芽脂溶性部位中11个有效成分的定量分析方法,为该部位的后期研究和开发利用奠定了可靠基础。
Abstract
OBJECTIVE To make out the relative contents of active components in the hydrophobic parts of Artemisia integrifolia. METHODS The contents of (+)-(S)-dihydro-ar-curcumene(1), (+)-(S)-ar-curcumene (2), (+)-(S)-dihydro-ar-turmerone(3), (+)-(S)-ar-turmerone (4), eugenol (5) palmitic acid (6),linoleic acid (7), linolenic acid (8), 12,13-epoxylinolenic acid (9), oleic acid (10) and chamazulene (11) were determined by gas chromatography. RESULTS Under the selected GC conditions, the linear ranges of compounds 1, 2, 4, 5 and 8 were 5.0-100.0 μg·mL-1(r＞0.999 3), and those of the compounds 3, 6, 7, 9, 10 and 11 were 1.0-50.0 μg·mL-1(r＞0.999 4). The recoveries of compounds 1-11 were 97.8%(RSD=1.7%), 96.3%(RSD=1.8%), 97.0%(RSD=1.8%), 96.7%(RSD=1.7%), 98.2%(RSD=1.8%), 96.8%(RSD=1.9%), 96.3%(RSD=1.8%), 97.3%(RSD=1.9%), 97.1%(RSD=2.0%), 97.2%(RSD=1.7%) and 97.7%(RSD=1.7%), respectively. CONCLUSION The quantitative analysis method for 11 active components in the hydrophobic parts of Artemisia integrifolia is established, which lays a reliable foundation for the later research and development of the part.
关键词
柳蒿芽 /
脂溶性部位 /
气相色谱法 /
萜类 /
苯丙素 /
脂肪酸类 /
含量测定
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Key words
Artemisia integrifolia /
hydrophobic parts /
GC /
terpene /
phenylpropanoid /
fatty acid /
content determination
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中图分类号：
R284
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参考文献
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脚注
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基金
内蒙古自治区蒙医药标准化项目资助(MB017);内蒙古自治区科技计划项目资助(2019GG118)
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